Iron-molybdenum cofactor from nitrogenase. Modified extraction methods as probes for composition.
نویسندگان
چکیده
منابع مشابه
Biochemistry Metal and sulfur composition of iron - molybdenum cofactor of nitrogenase ( molybdenum - iron protein / dinitrogen reduction / iron - sulfur clusters )
The sulfur content of N-methylformamide solutions of cofactor from Clostridium pasteurianum nitrogenase has been determined to be 11.9 (+0.9) mol per mol of molybdenum. This number was determined radiochemically, using iron-molybdenum cofactor isolated from molybdenum-iron protein from bacteria grown on 35SO4. A total of 3.2 (±0.2) mol of sulfur per mol of molybdenum was found to be present in ...
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Klebsiella pneumoniae accumulates molybdenum during nitrogenase derepression. The molybdenum is primarily in nitrogenase component I in the form of iron-molybdenum cofactor (FeMo-co). Mutations in any of three genes (nifB, nifN, and nifE) involved in the biosynthesis of FeMo-co resulted in very low molybdenum accumulation and in a molybdenum-free nitrogenase component I. A mutant lacking both s...
متن کاملIn vitro synthesis of the iron-molybdenum cofactor of nitrogenase.
Molybdate- and ATP-dependent in vitro synthesis of the iron-molybdenum cofactor (FeMo-co) of nitrogenase requires the protein products of at least the nifB, nifN, and nifE genes. Extracts of FeMo-co-negative mutants of Klebsiella pneumoniae and Azotobacter vinelandii with lesions in different genes can be complemented for FeMo-co synthesis. Both K. pneumoniae and A. vinelandii dinitrogenase (co...
متن کاملBiosynthesis of the Iron-Molybdenum Cofactor of Nitrogenase*
The iron-molybdenum cofactor, or the Mcluster, serves as the active site of molybdenum nitrogenase. Arguably one of the most complex metal cofactors in biological systems, the M-cluster is assembled through the formation of an 8Fe core prior to the insertion of Mo and homocitrate into this core. Here, we review the recent progress in the research area of M-cluster assembly, with an emphasis on ...
متن کاملBiosynthesis of the iron-molybdenum cofactor of nitrogenase.
The iron-molybdenum cofactor (FeMo-co) of nitrogenase is a unique molybdenum-containing prosthetic group that has been proposed to form an integral part of the active site of dinitrogenase. In Klebsiella pneumoniae, at least six nif (nitrogen fixation) gene products are required for the biosynthesis of FeMo-co, including NIFB, NIFNE, NIFH, NIFQ, and NIFV. An in vitro system for the synthesis of...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1982
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)34294-7